Journal: PLoS ONE

Article Title: Repurposing a microfluidic formulation device for automated DNA construction

doi: 10.1371/journal.pone.0242157

Figure Lengend Snippet: User requests are sent from the TeselaGen DESIGN module to j5 to generate a scar-less combinatorial Golden Gate DNA assembly protocol. The automation module allocates resources, and initiates construction. Gel-purified, BsaI and DpnI-digested PCR products are generated off-chip according to the protocol, and then supplied to and then assembled together on the microfluidic chip. Assembled DNA (plasmids) are transformed into a host system of interest ( E . coli ) off-chip. The assembled DNA can then be sequence validated, and resulting reports generated.

Article Snippet: We demonstrated on-chip, TeselaGen-designed, Golden Gate DNA assembly of DNA assembly pieces, and sequence and functionally validated the resulting DNA constructs off-chip via colony PCR, Sanger sequencing, and micro-plate reader assays.

Techniques: Purification, Generated, Transformation Assay, Sequencing

Journal: PLoS ONE

Article Title: Repurposing a microfluidic formulation device for automated DNA construction

doi: 10.1371/journal.pone.0242157

Figure Lengend Snippet: A . Schematic of combinatorial assembly of pre-made DNA assembly pieces using the Golden Gate method on the microfluidic chip. B . A screenshot of the TeselaGen DESIGN module (classic view)–design of the combinatorial library: a vector backbone (first column), two promoters (second column), and two (bicistronic) ribosomal binding sites followed by gfp (third column).

Article Snippet: We demonstrated on-chip, TeselaGen-designed, Golden Gate DNA assembly of DNA assembly pieces, and sequence and functionally validated the resulting DNA constructs off-chip via colony PCR, Sanger sequencing, and micro-plate reader assays.

Techniques: Plasmid Preparation, Binding Assay